The Cutadapt source code is on GitHub. Cutadapt is written in Python 3 with some extension modules that are written in Cython. Support for Python 2 has been dropped.

Development installation

For development, make sure that you install Cython and tox. We also recommend using a virtualenv. This sequence of commands should work:

git clone  # or clone your own fork
cd cutadapt
python3 -m venv venv
venv/bin/pip3 install Cython pytest nose tox
venv/bin/pip3 install -e .

Then you can run Cutadapt like this (or activate the virtualenv and omit the venv/bin part):

venv/bin/cutadapt --help

The tests can then be run like this:


Or with tox (but then you will need to have binaries for all tested Python versions installed):


Making a release

Since version 1.17, Travis CI is used to automatically deploy a new Cutadapt release (both as an sdist and as wheels) whenever a new tag is pushed to the Git repository.

Cutadapt uses setuptools_scm to automatically manage version numbers. This means that the version is not stored in the source code but derived from the most recent Git tag. The following procedure can be used to bump the version and make a new release.

  1. Update CHANGES.rst (version number and list of changes)

  2. Ensure you have no uncommitted changes in the working copy.

  3. Run a git pull.

  4. Run tox, ensuring all tests pass.

  5. Tag the current commit with the version number (there must be a v prefix):

    git tag v0.1

    To release a development version, use a dev version number such as v1.17.dev1. Users will not automatically get these unless they use pip install --pre.

  6. Push the tag:

    git push --tags
  7. Wait for Travis to finish and to deploy to PyPI.

  8. The bioconda recipe also needs to be updated, but the bioconda bot will likely do this automatically if you just wait a little while.

    Ensure that the list of dependencies (the requirements: section in the recipe) is in sync with the file.

If something went wrong after a version has already been tagged and published to PyPI, fix the problem and tag a new version. Do not change a version that has already been uploaded.


Contributions to Cutadapt in the form of source code or documentation improvements or helping out with responding to issues are welcome!

To contribute to Cutadapt development, it is easiest to send in a pull request (PR) on GitHub.

Here are some guidelines for how to do this. They are not strict rules. When in doubt, send in a PR and we will sort it out.

  • Limit a PR to a single topic. Submit multiple PRs if necessary. This way, it is easier to discuss the changes individually, and in case we find that one of them should not go in, the others can still be accepted.
  • For larger changes, consider opening an issue first to plan what you want to do.
  • Include appropriate unit or integration tests. Sometimes, tests are hard to write or don’t make sense. If you think this is the case, just leave the tests out initially and we can discuss whether to add any.
  • Add documentation and a changelog entry if appropriate.

Code style

  • Cutadapt tries to follow PEP8, except that the allowed line length is 100 characters, not 80. But try to wrap comments after 80 characters.
  • There are inconsistencies in the current code base since it’s a few years old already. New code should follow the current rules, however.
  • At the moment, no automatic code formatting is done, but one idea might be to switch to the black code formatter at some point. If you’re familiar with its style, you can use that already now for new code to make the diff smaller.
  • Prefer double quotation marks in new code. This will also make the diff smaller if we eventually switch to black.
  • Using an IDE is beneficial (PyCharm, for example). It helps to catch lots of style issues early (unused imports, spacing etc.).
  • Avoid unnecessary abbreviations for variable names. Code is more often read than written.
  • When writing a help text for a new command-line option, look at the output of cutadapt --help and try to make it look nice and short.
  • In comments and documentation, capitalize FASTQ, BWA, CPU etc.